pej2gfp puro (Addgene inc)
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Addgene inc
pej2gfp puro
Pej2gfp Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pej2gfp+puro/EJ2GFP-puro+(Plasmid+%2344025)/us11359200-323-15-17
Average 93 stars, based on 35 article reviews
Pej2gfp Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pej2gfp+puro/EJ2GFP-puro+(Plasmid+%2344025)/us11359200-323-15-17
Average 93 stars, based on 35 article reviews
pej2gfp puro - by Bioz Stars,
2026-10
93/100 stars
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Non-Homologous End Joining:Article Title: Cancer treatment by MALAT1 inhibition Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Article Title: Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and apoptosis in multiple myeloma Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Homologous Recombination:Article Title: Cancer treatment by MALAT1 inhibition Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Article Title: Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and apoptosis in multiple myeloma Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Stable Transfection:Article Title: Cancer treatment by MALAT1 inhibition Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Article Title: Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and apoptosis in multiple myeloma Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Transfection:Article Title: Cancer treatment by MALAT1 inhibition Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, Article Title: Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and apoptosis in multiple myeloma Article Snippet: Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific). .. To establish A-NHEJ, NHEJ and homologous recombination (HR) DNA repair pathway reporter stable cell lines, |
![Inhibition of DNA repair by knockdown of PHAX. ( A ) U2OS cells were transfected with the indicated siRNAs, followed by UV-irradiation (20 J/m 2 ). After 6-h incubation, phase-contrast images were obtained. ( B , C ) U2OS cells were transfected with the indicated siRNAs, followed by ADR ( B ) or CPT ( C ) at the indicated concentrations. After 24-h incubation, cell viabilities were determined by alamarBlue assay. ( D ) U2OS cells were treated as in A . The indicated protein levels were determined by western blotting analysis. ( E – H ) U2OS cells were cotransfected with the indicated siRNAs and each reporter plasmid for DNA repair assay (pDRGFP for HDR [ E , F ] or <t>pEJ2GFP</t> for NHEJ [ G , H ]). Fluorescence microscopic images were obtained, and the numbers of GFP-positive cells were counted. As a positive control, the cells were treated with PARP inhibitor (PARPi) at 100 nM for 48 h. Data are the means ± S.D. ( n = 4). (*) P < 0.01, (**) P < 0.001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6570/pmc07566570/pmc07566570__1716f01.jpg)